Soybean Rust 
Alan Henn, Extension Plant Pathologist
Mississippi State University
May 30, 2004
Soybean Rust is one of nine agricultural select agents/toxins (Table 1) designated by congress in the Homeland Security Act of 2002. If introduced into the United States, these agents are thought to be capable of significantly damaging agriculture. Plant pathogens highlighted in yellow (Table 1) can thrive under our climatic conditions and are potential threats to Mississippi agriculture, or in the case of citrus (highlighted in green), Mississippi may provide a bridge for the disease to enter into Florida or Louisiana/Texas.

Soybean rust is not in the United States yet, and there is no reason to panic. What needs to be done is to prepare for its eventual arrival by learning all we can about the disease and how to control it before it arrives. The purpose of this page is to provide access to this information and advise Mississippi growers on how to manage it when it does arrive. This information is provided to you by Mississppi State University Extension and Agricultural Station personnel. Please send comments and suggestions to alan henn, Extension Plant Pathologist.

Table 1. Select Agents /toxins from the Agricultural Bioterrorism Protection Act of 2002.

Pathogen

Disease

Primary host

Librobacter africanus and L. asiaticus

Huanglongbing/greening phytoplasma disease

Citrus

Peronosclerospora philippinensis

Philippine downy mildew

Corn

Phakopsora pachyrhizi

Soybean rust

Soybean, other legumes

Xanthomonas oryzae pv. oryzicola

Bacterial leaf streak

Rice

Ralstonia solanacearum race 3 biovar 2

Bacterial wilt, brown rot

Many hosts, esp. solanaceous

Sclerophthora rayssiae var. zeae

Brown stripe downy mildew

Corn

Plum pox potyvirus

Sharka, Plum pox

Stone fruits

Synchytrium endobioticum

Potato wart or potato canker

Irish potato

Xylella fastidiosa

Citrus variegated chlorosis

Citrus




Soybean Rust: history
and spread 


Soybean rust is caused by two different species of the fungus Phakopsora (pronounced fa cop soar a). One species, P. pachyrhizi, devastates soybean yields, whereas the other species, P. meibomiae, has little impact on soybeans.

P. meibomiae is found in the Carribean and Central and South America. Because of its close proximity to the United States, it is possible that it will be introduced into U.S. soybean production. Because P. meibomiae causes few problems on soybeans, its introduction would not be a problem if it were not so difficult to tell from P. pachyrhizi. A difficulty that has led to confusion elsewhere. To alleviate this confusion and to advance our understanding of the disease, USDA-ARS contracted with the Joint Genome Institute (JGI) to sequence the genomes of both Phakopsora species. The sequence data is to be made public. These data should facilitate methods to distinguish between the two species of Soybean rust and among races of P. pachyrhizi. Information on this initiative can be found at http://www.jgi.doe.gov/ .

P. pachyrhizi was first discovered in Southeast Asia and has spread - rapidly at times - through Africa and South America (Figure 1). Some reported yield losses in various countries which it has infested appear in Table 2.

In the western hemisphere, Soybean rust arrived in Paraguay in 2001. By 2002 it had spread to neighboring Brazil and Argentina. In 2003 it infested most of the major Brazilian soybean production areas and was discovered in Boliva. As of December 2003, Soybean rust was present in 70% of the norther soybean crop area and in 50% of the eastern soybean crop area.

In Brazil this year, there has been continued cultivation of soybeans because of good market price. Thus, there was a continuous source of P. pachyrhizi spores. Late 2003 plantings were showing infections at growth stages V3-V4, about 20-25 days after planting.

So far, P. pachyrhizi has not been shown to exist in the northwestern hemisphere - above the equator. Once north of the equator, the combination of intertropical convergence zone and tropical storms will probably move the rust spores into North America.

Spread of Soybean Rust and where it might be a problem in the United States
Much forecasting work (epidemiology) on soybean rust in the United States has been conducted by Dr. X. B. Yang. Dr. Yang, was formerly with the USDA, Foreign Agricultural Service, and is now at Iowa State University (http://www.ag.iastate.edu/departments/plantpath/faculty_pages/xbyang.html ). During the mid 1990s he became familiar with the devastating effect of Soybean rust on Chinese soybean production. A map of the distribution of Soybean rust in China appears in Figure 2. Its occurrence varies with the location and year (Figure 2).

In China, the latitude line of 35 degrees north is the limit of frequent SBR disease occurrence (Figure 2). China experiences frequent, severe epidemics in its south (below 30° north in latitude), frequent but not always severe epidemics in the center of the country (between 30 and 37°), and occasional but mild epidemics in the north (above 37°). Areas south of this line are frequently affected by SBR, while areas north of the line may be affected by SBR but only occasionally. In the U.S., the 35 degree latitude line passes through North and South Carolina, the southern border of Tennessee, Arkansas, and Oklahoma. While other epidemiological considerations will change this observation in the U.S., it may give us some idea of what to expect.

Introduction of the pathogen into the U.S. may be purposeful (bioterrorism), by accident, or by wind currents. The movement of P. pachyrhizi spores on wind currents is being studied and modeled. Please refer to http://soybeanrust.zedxinc.com for further information on the spread of P. pachyrhizi by winds (aerobiology). A power point presentation may be found on the USDA APHIS wet site http://www.aphis.usda.gov/ppq/ep/soybean_rust/rsampling5.pps.

In the U.S., entrance of P. pachyrhizi is expected somewhere around the gulf coast, an environment closer to Africa and South America and more suitable to the pathogen than other regions of the U.S. In Africa, several years passed before SBR reached it full expected range (Figure 1), arriving in South Africa in 2001. Opinion varies on the time required for SBR to dominate its expected range in the U.S., but several years would likely be required for SBR to attain its full intensity and range. Figure 3 shows the spread of Southern corn leaf blight through the United States in 1981 (Moore et. al. 1981). It is possible that soybean rust may spread like Southern corn leaf blight.

Conditions required for Soybean rust include favorable temperatures and humidities for spores to germinate, for infection of the host and for the developing uredinia to produce spores. Researchers have investigated these requirements, and some found by Dr. X. B. Yang are in Table 3.

It appears that non-optimal higher temperatures may be overcome by longer periods when the leaves are wet (e.g. dew periods). Extensive periods of leaf wetness, almost nightly, occur in the lower canopy of soybeans in the Mississippi delta. Temperatures themselves, are not unfavorable (http://www.srh.noaa.gov/jan/climate_connection.htm). Such conditions, and an abundance of alternative hosts (see below) indicate that the Mississippi delta will probably be a good incubator for soybean rust.

These data and much more scouting information are summarized in an informative CD created by Dr. X. B. Yang and available from the Crop Advisor Institue ( http://www.cai.iastate.edu ). Mississippi State Extension Service employees may check out a copy from Kathy Nash.

Table 3. Temprature (degrees F) for P. pachyrhizi to germinate and infect. Data from Yang et al. 1991.

Stage of P. pachyrhizi development

Temperature

range

Temperature

optimum

Moisture

Spore germination

46-97

61-75


Infection

52-82

66-75

>= 6 hour dew

Uredinia maturation


72-81



Soybean rust symptoms  


P. pachyrhizi may cause different colors and severity of lesions, depending upon the genetics of the soybean host. The lesions are characterized as either red/brown or tan. Examples of these symptoms may be found on the USDA APHIS web site: http://www.aphis.usda.gov/ppq/ep/soybean_rust/detection5.html

Tan lesions contain small uredinia (generally a raised pustule like area) surrounded by slightly discolored areas. The young uredinia have a small hole (ostiole) in their upper surface from which the spores (urediniospores) emerge. As the uredinia grow, many tan colored urediniospores are released, giving the surface the appearance of light brown to white raised areas .

Reddish/brown lesions cause a larger lesion (dead area) on the surface of the leaf, but contain few uredinia that usually have few spores visible on the leaf surface .

Early lesions may be confused with bacterial pustule (Xanthomonas campestis pv. phaseoli) or Bacterial blight (Psuedomonas glycinea) or brown spot (Septoria glycines). The bacterial diseases usually occur on the underside of the leaves and produce a raised light brown blister within the lesion. The lesions may vary from small specks to large irregular areas. If no bacteria or spores are present, the early bacterial/Soybean rust lesions may be difficult to distinguish from Soybean rust. Spores will almost always be present.

In older bacterial lesions may be distinguished from Soybean rust by two characteristics that may be seen with a powerful hand lens or microscope. The bacteria emerge through a torn crack in the leaf epidermis whereas P. pachyrhizi spores emerge through the small roundish ostioloe. In the case of more advanced Soybean rust lesions, white clumps of spores catch on top of the raised area in the lesion. Sometimes these will be in columns. Spores will be present. Further identification needs to be made by other means, including microscopic and by PCR (Polymerase Chain Reaction).

Spores can be detected by wiping a clean white tissue, such as Kleenex, on the suspect area. If a tan or brown smear is seen on the tissue than spores are probably present.

The affect of Soybean rust on the soybean plant

Soybean rust affects the plant by causing:
	-Premature defoliation
	-Increase in number of unfilled pods/plant
	-Decrease in number of normal pods/plant
	-Decrease in number of seeds/plant
	-Decrease in weight of seed/plant
	-Decrease in 1000-seed weight
	-Decrease in seed germination

P. pachyrhizi infects all above-ground parts of the soybean plant. Rust pustules may be found on both sides of the leaves, the stems, pods, and petioles. Although it may infect the soybean plant as early as the cotyledon stage, it does not start to cause damage until the plant has started to flower. It is speculated that changes in the flow of plant nutrients from vegetative tissues (leaves and stems) to reproductive tissues such as flowers, pods and seeds induces increased susceptibility.

Under conducive environmental conditions, the infection may move quite rapidly, proceeding from spore through infection to producing new spores within a few days. Earliest lesions appear where moisture remains the highest for the longest period - lower leaves of the plant, especially near low/wet areas such as ditches or along roads and streams.

Many questions remain unanswered - can we tolerate defoliation inside the canopy while retaining the canopy leaves? How much defoliation - if any- can be tolerated? Will our indeterminate cultivars behave differently than determinate cultivars (more discussion under control).

Genetic Resistance to Soybean Rust
Active screening of soybean germplasm has been conducted by the USDA in other countries since the 1980's. Screening continues at Ft. Dietrick, in a quarantine lab. Additional screening programs are being conducted by other countries, including Zimbabwae. Promising lines have been identified by several programs, only to fail after one to several years in the field. Apparently new races of P. pachyrhizi which can infect previously reistant lines develop rapidly.

The Fort Dietrik program is screening soybean germplasm against a number of P. pachyrhizi races simultaineously. They are going to reexamine a few lines, but apparently no notable resistance has been found. There does seem to be some good resistance in some soybean relatives, but none of this has been integrated into agronomically acceptible lines.

Breeding work needs to continue at full pace to provide a long term solution to Soybean rust. In the meantime, chemical control is the only known alternative.

Alternative hosts
P. pachyrhizi does not seem to be a typical rust. Most rusts have five life stages:

     Stage 0	Spermagonia bearing spermatia (n) and receptive hyphae (n)
     Stage I	Accia bearing acciospores (n+n)
     Stage II	Uredinia bearing urediniospores (n+n)
     Stage III	Telia bearing teliospores (n+n -> 2n)
     Stage IV	Basidia bearing basidiospores (n)
Only uredinia spores have been found for P. pachyrhizi. While teliospores have been found mixed with uredinia below the leaf epidermis, none have been shown to germinate (be viable) (NPAG data, 2002 draft). Additionally, most rusts alternate hosts amongst each of the stages and infect very few hosts in any particular stage (usually only one). In contrast, the uredinial stage of P. pachyrhizi infects many hosts (Table 4). Because it is the uredial spore that infects many hosts, and not other spore types, the term "Alternative" hosts, not alternate hosts is used.

Most alternative hosts are in the Legeume family Papilionoideae. This host list will probably increase with new research.


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Table 4.  Known hosts of P. pachyrhizi, the pathogen causing soybean rust. 1

Alysicarpus glumaceus Alyce clover (Naturalized in West Indies and FL?) #
Alysicarpus vaginalis - ***
Cajanus sp. pegeon pea relative
Cajanus cajan Cajan, pegeon pea Widely cultivated in tropical countries - * 
Calopogonium muconoides
Canavalia gladiata Gian butterbean, Sword Bean - C
Canavalia maritima
Cassia occidentalis - *
Centrosema pubescens Butterfly pea;  W. Indies and Mexico - #
Clitoria ternatea - #
Coronilla varia - ***
Crotalaria anagyroides Rattlebox Tropical northern South America #
Crotalaria dissaromoensis
Crotalaria linifolia
Crotalaria pallida
Crotalaria spectabilis - **
Delonix regia Royal Poinciana Wide-branching tree, indoor cultivated
Desmodium discolor - # 
Desmodium rhytidophyllum - #
Desmodium triflorum - #
Desmodium varians - # 
Dolichos axillaris - #
Glycine argyrea
G. canescens Soybean relative
G. clandestina Soybean relative
G. curvata
G. cyrtoloba
G. falcata, Soybean relative
G. latifolia
G.latrobeana Soybean relative
G. max Soybean Major agricultural crop in US*
G. microphylla
G. soja
G. tabacina
G. tomentella Soybean relative
Hardenbergi violecea
Kennedia coccinea
Kennedia prostrata
Kennedia rubicunda
Kudzu, Pueraria lobata or Pueraria phaseoloides - name change*
Kummerowia striata
Lalab purpureus Hyacinth bean (Dolichos  lablab) - C
Lespedeza bicolor - ***
Lespedeza juncea
Lotus americana -  # C?
Lotus major
Lotus purshianus
Lupinus albus - C
Lupinus angustifolius
Lupinus hirsutus Blue lupine (Annual; southern Europe)
Lupinus luteus
Macroptilium atropurpureum Siratro; purple bean Grows wild in Cen. And S. America
Macroptilium bracteatum
Macroptilium lathyroides
Macrotyloma axillare 
Medicago arborea Medic (Shrub; southern Europe) - #
Melilotus officinalis Yellow sweet clover Eurasia; naturalized in N. America - - ***
M. Speciosus 
Mucuna cochinchinesis Velvetbean relative - C
Neonotonia (Glycine) wrightii Glycine (Old World probably)
Pachyrhizus erosus Yam bean; jicama C. America; naturalized in FL C
Phaseolus coccincus Scarlet runner bean - C
Phaseolus lunatus Butter bean, lima bean Tropical SA: important edible bean*
P. vulgaris Kidney bean; green bean Tropical America; widely cultivated*
Pisum sativum - garden peas
Psophocarpus tetragonolobus
Psoralea tenax - #
Pueraria lobata  or Pueraria phaseoloides - name change* Kudzu 
Rhynchosia minima  Creeping tropical weed #
Sesbania exaltata Colorado River hemp NY to FL; west to southern CA*
S. sericea
S. vescaria - *
Teramnus uncinatus
Trifolium incarnatum - *
T. repens - *
Trigonella foenum-gracecum Fenugreek (Asia & southern Europe; forage)
Vicia dasycarpa - ***
Vicia faba - C
Vigna luteola - ** coastal area
Vigna mungo
Vigna radiata
Vigna unguiculata Cowpea, black-eyed pea Widely planted in warm regions*

______________________________________________________
# Closely related species present in the state
C Cultivated in the state as an ornamental
*Frequent in the State
**Occasional in the State
***Infrequent in the State
1 host list adapted from USDA-APHIS Strategic plan to minimize the impact of the introduction and establishment of soybean rust on soybean production in the United States: Soybean rust: Phakopsora pachyrihizi, P. meibomiae, and "Status of Scientific Evidence on Risks Associated with the Introduction into the Continental United States of Phakopsora pachyrhizi with Imported Soybean Grain, Seed and Meal", dated 23 Feb. 2004

Dr. Victor Maddox, GeoResources Institute, MSU, helped identify those plants with closely related species in Mississippi.

Compiled by Alan Henn, Extension Plant Pathologist, Mississippi State Univeristy, 662.325.4535.

________________________________________________________________________

The USDA in its report "Status of Scientific Evidence on Risks Associated with the Introduction into the Continental United States of Phakopsora pachyrhizi with Imported Soybean Grain, Seed and Meal", dated 23 Feb. 2004 www.aphis.usda.gov/ppq/ep/ soybean_rust/sbr_riskevidoc2_23_04.pdf notes that P. pachyrhizi has been naturally found on Crotalaria spp., Desmodium spp., Glycine spp., Dennedia spp., Lablab purpureus, Lupinus spp., Macroptilium spp., Melilotus officinalis, neonotonia wightii, Pachyrhizus crosus, Phaseolus spp, Pueraria lobata, Sesbania exaltata, Trifolium incarnatum, Vicia dasycarpa, Vigna spp.

Note that Kudzu (Pueraria lobata or Pueraria phaseoloides) is an alternative host for P. pachyrhizi. It produces many spores on Kudzu but does little damage to it otherwise. In Brazil, where cold weather does not kill the Kudzu, rust from Kudzu infects soybeans early in their culture. In Paraguay, soybeans growing close to infected kudzu were showing rust symptoms this past year at the V3-V4 growth stages or 20-25 days after sowing.

Kudzu overwintering on the coast, may provide an inoculum source in Mississippi. Rust may also hurt the culture of various beans and peas and those in turn may provide additional inoculum to infest later planted soybeans.

P. pachyrhizi infects the alternative hosts kudzu, sickle pod, sweet peas and pigeon peas up to one month prior to soybeans in Brazil. While they may act as a indicator for the presence of P. pachyrhizi, they also serve an inoculum reservoir.

Control of soybean rust
The best long term hope for management of Soybean rust is resistant plant cultivars. None are available today, and probably won't be for another 4-5 years. The sequencing project may help in the development of transgenic cultivars.

Management techniques that have been investigated in other countries include planting dates, row spacing and use of soybeans in different maturity groups.

Changing planting dates has not worked in Zimbabwae. Climatic conditions caused the plants to enter the reproductive stages at similarl times, despite the various planting dates.

Changing plant spacing (inter-row and densities) has shown no benefit in South Africa, but using different maturity groups may be helpful. In Brazil, narrow soybean row widths have been abandoned in favor of the relatively wide rows between (45 to 50 cm). This allows better penetration of the soybean canopy by soybean fungicides as well as avoiding a microenvironment conducive to soybean rust.

A key factor in controlling soybean rust is the timing of the first fungicide application. Experience in both Zimbabwae and Brazil has shown that early warning is vital. Some countries have established a network of sentinel soybean plantings (each about 0.5 A) around the country. The sentinel crops are planted about one month prior to the commercial crops and scouted daily by the cooperator.

Because of their greater maturity, these sentinel crops become infected first, giving area growers warning to start fungicide applications. Some of these sentinel crops are then turned into fungicide demonstration plots.

This season in Mississippi, the SMART program, led by Dr. Alan Blaine, in association with Dr. Gabe Sciumbato of the Mississippi State Delta Research and Extension Center, have established sentinel crops around the state. There was no funding to do this, the expense coming out of their operations monies. These plots are being monitored at least once/week.

The Brazilians also have not defined a particular growth stage to start spraying. Rather, the first spray depends on when the first infection is detected, on weather conditions, and on the mode of action of the fungicide used (protective or protective/curative).

For indeterminate soybeans, timing of applications in relation to growth stage is much more difficult because the plants continue growing and putting out new leaves and flowers. Thus, indeterminate soybeans may require more sprays with shorter intervals between sprays, because new leaves may be exposed to infection every week.

Fungicidal management
Fungicides have become the management tool of first choice in most countries. Dr. Clyde Levy in Zimbabwae determined that full-canopy spray coverage is essential. All reproductive growth needs to be protected. The fungicide needs to protect both the upper trifoliates and kill the spores in the lower and middle canopy.

Experience in both Zimbabwae and Brazil has shown that spray skips become rapidly diseased, and very noticeable. Severe localized yield losses result.

In Brazil short season varieties require fewer applications (one) than longer season varieties (2-3). During the 2003-2004 Brazilian growing season, it is estimated that 95% of the acreage required 1.5 – 2 sprays, but selected fields received as many as four. Despite these applications under conducive conditions for disease in Brazil, yield losses approximate 10%. Losses are 50% or greater without applications. As has been observed in Mississippi, Brazilian soybeans treated with fungicides remain green longer -extending the season.

Timing of fungicide applications is critical to effective disease control. In Zimbabwe: the recommendation developed is to apply the first spray at first flowering (R1 growth stage, approximately 50 days after planting), a second spray applied 20 days later, and a third 20 days thereafter. Generally three sprays work best, but two may be adequate under low disease pressure. Spraying earlier during reproductive growth is more beneficial than delayed applications.

In Brazil: Full canopy spray coverage is required. Application is made at first detection of soybean rust in the region as indicated by the sentinel crops. The disease normally intensifies during the reproductive phase, resulting in a spray around R1. A second spray is applied 15-20 days later for protective fungicides. If needed a third spray is made 20-25 days later for protective/curative fungicides, and other sprays are made if necessary. Because early identification can be difficult, many are moving to applications based on soybean growth stage.

Application methods are diverse in countries infested with Soybean rust. Large growers in Brazil generally rely on aerial applications of ultra low volumes in oil. Spray volume is usually less than 10 liters/hectare of which 3.5 liters are oil, 1.5 liters water and the rest fungicide product ( ). Ground applicators are generally agreed to be more effective. Most applications in Brazil are made in 70-75 liters of water/hectare ( ). Cone nozzles are preferred.

Adjuvants have been used with some fungicide products. They have been found to help sprays penetrate the canopy to the lower leaves.

In general, growers in most countries prefer triazole and strobilurin class fungicides (links here to examples) because of their systemic activity. The particular fungicide group used depends upon the developmental stage of the plant and whether or not it has already been infected by rust. Generally, but not always, the triazole group of fungicides have an 'azole' near the end of the chemical name of the active ingredient whereas strobilurins molst often have a 'strobin'. Both groups of fungicides penetrate into the plant tissue and provide protection against new infection, but some of the triazoles also have some 'curative' ability - able to destroy some of the fungus already infecting the plant.

Some protectant fungicides are also used. If they are, they are often applied in late V5 to protect the lower foliage. This is then followed by a systemic fungicidal applications during R1-R5. Most recently Brazilian growers have started their initial application using fungicides formulated as mixtures of both a triazole and a strobilurin. This application is followed 20-25 days later by either a triazole or a strobilurin, depending upon infection status of the field.

Over the six years since SBR's introduction into Zimbabwe, several triazole fungicides, a benzimidazole + triazole mix, and a morpholine have been utilized without any evidence of pathogen resistance. The fungicides registered are carbendazim + flusilazole, triadimenol, cyproconazole, tebuconazole, difenoconazole, propiconazole, and triforine - the first two having been used extensively.

Fungicides recommended for control of soybean rust in Brazil include penetrant fungicides applied prior to disease establishment. These are applied at repeated at 10-15 day intervals, if needed. This group of fungicides includes difenoconazole, azoxystrobin and fluquinconazole.

A second group of fungicides are used either before or after disease establishment, since they have some curative ability. These must be reapplied at 20-25 days intervals. This group of curative chemicals always includes a triazole. Active ingredients include epoxyconazole + pyraclostrobin, tebuconazole, flutriafol, azoxystrobin + cyproconazole, trifloxystrobin + propiconazole and trifloxystrobin + cyproconazole.

U.S. fungicides
The USDA has been conducting fungicide tests in other countries. Some of these results may be found at http://www.ipmcenters.org/NewsAlerts/soybeanrust/

Several fungicides in the United States already have labels for Soybean rust, Quadris (azoxystrobin) (strobilurin class), Echo (chlorothalonil, a non-penetrating surface protectant needing reapplication every 7-14 days). BASF has applied for a regular label for Headline, containing pyraclostrobin.

The experience of growers in other countries have shown that these fungicides alone are not sufficient, nor will enough quantities be available to meet needs. To meet the expected market demand and needs for fungicides, Section 18, are being applied for by states.

Section 18
To meet crop protection needs, a special needs request called a "Quarantine Exemption (Section 18)" has been submitted by Minnesota and South Dakota to manage Soybean rust on soybeans when it arrives. This submission, has been approved, and serves as a "master" or "generic" application that other states can reference. The Section 18 application can be accessed at: http://plantsci.sdstate.edu/draperm/SoybeanRustSection18/

Mississippi can add its name to the soybean rust Section 18 by making a formal request to EPA in which they reference the MN/SD application and supply a limited amount of state specific information.

The Section 18 will be activated when Soybean rust is first identified in the United States. It will be active for three years.

Fungicides (trade names in parentheses) listed in the Section 18 are propiconazole (Tilt, Propimax, Bumper), tebuconazole (Folicur), myclobutanil (Laredo), trifloxystrobin + propiconazole (Stratego), tetraconazole (Eminent), pyraclostrobin (Headline), and boscalid + pyraclostrobin (Pristine).

The active ingredients propiconazole and myclobutanil have been approved for immediate use if Soybean rust were found today. The other active ingredients are still under EPA review.

Mississippi Department of Agriculture, Bureau of Plant Industry Tommy McDaniels and Extension Plant Pathologist alan henn are working on this for the state. A copy of the state application may be found as a Microsoft Word document here; EPA's approvals (in PDF format) are here for propiconazole, myclobutanil and tebuconazole.

Web resources
APHIS SBR site and strategic plan - http://www.aphis.usda.gov/ppq/ep/soybean_rust/
APHIS risk maps - http://soybeanrust.zedxinc.com & http://aries.zedxinc.com/sbrust.php  
Florida SBR Pest Alert - http://www.doacs.state.fl.us/%7Epi/enpp/pathology/soybeanrust.html
Illinois facts about SBR - http://www.ag.uiuc.edu/cespubs/pest/articles/200213k.html
Iowa State University Crop Advisor Institute module on SBR - http://www.cai.iastate.edu
IPM Centers SBR website (past Working Group meeting summaries, membership, action plan, SBR
    fungicide efficacy trials, and useful links) - http://www.ipmcenters.org/NewsAlerts/soybeanrust/
National Pest Alert (States & USDA) - http://www.ncpmc.org/soybeanrust
NC504 SBR Committee - http://www.lgu.umd.edu/project/home.cfm?trackID=3154
Ohio SBR facts - http://ohioline.osu.edu/ac-fact/0048.html
Quarantine Exemption (Section 18) - http://plantsci.sdstate.edu/draperm/SoybeanRustSection18
United Soybean Board SBR guide - http://www.unitedsoybean.org/soybeanrustguide.pdf
Aerobiology of Soybean rust and risk assessment http://soybeanrust.zedxinc.com  
Aerobiology of Soybean rust and risk assessment  http://aries.zedxinc.com/sbrust.php  
Michigan State University Information  http:///www.pested.msu.edu/